Encyclopedia

SDS polyacrylamide gel electrophoresis

SDS polyacrylamide gel electrophoresis

[¦es¦dē¦es ‚päl·ē·ə¦kril·ə·mīd ‚jel i‚lek·trō·fə′rē·səs]
(cell and molecular biology)
An electrophoretic technique in which proteins are denatured by the negatively charged detergent sodium dodecyl sulfate, which masks their intrinsic electrical charge so that when the constituent polypeptide chains are run through a polyacrylamide gel, the protein molecules are separated according to size, not electrical charge. Abbreviated SDS-PAGE.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
Mentioned in
References in periodicals archive
The mechanism of silver staining of proteins separated by SDS polyacrylamide gel electrophoresis. Biotech Histochem 2017; 92(2): 1-7.
AN IMPROVED MODIFIED PROTOCOL FOR SILVER STAINING OF DS DNA IN AGAROSE GEL
This protein was found to be present in numerous cell cultures so I purified to homogeneity by preparative isoelectric focusing and SDS polyacrylamide gel electrophoresis this protein from human embryonic kidney cells (i.e., HEK293).
Comparison of the levels of the spicing factor PSF in individuals with dystonia
The SDS polyacrylamide gel electrophoresis (PAGE)[14] method was used for the determination of complexation concentration of beta-cyclodentrin ([beta]-CD) with insulin.
Betacyclodextrin complexed insulin loaded alginate microspheres--oral delivery system
Copyright © 2003-2025 Farlex, Inc Disclaimer
All content on this website, including dictionary, thesaurus, literature, geography, and other reference data is for informational purposes only. This information should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional.