APB-102 is being developed as a novel, one-time treatment of genetic (
SOD1) ALS.
Primer sequences for cumulus cell expansion -related and oxidative stress-related genes Gene Primer sequences Product size (bp) GAPDH 5'-GGGTCATCATCTCTGCACCT 176 5'-GGTCATAAGTCCCTCCACGA HAS2 5'-GGATCTCCTTCCTCAGCAGTGT 106 5'-ATTCCCAGAGGTCCGCTAATG TNFAIP6 5'-TGAAAGATGGGATGCATATTGC 101 5'-CATTTGGGAAGCCTGGAGATT PTX3 5'-CATGTATGTGAATTTGGACAACGA 101 5'-GCTTGTCCCACTCGGAGTTC PTGS2 5'-CTTAAACAAGAGCATCCAGAATGG 106 5'-GCTGTACGTAGTCTTCAATCACAATCT NRF2 5'-GATGGACTTGGAGCTGCCG 140 5'-GCTCATGCTCCTTCTGTCGT
SOD1 5'-CCACGTCCATCAGTTTGGAGA 92 5'-CTTTTGGCCCACCGTGTTTT CAT 5'-CTGGGACCCAACTATCTCCA 148 5'-GATGCTCGGGAGCACTAAAG GPX4 5'-GTGCTCGCTCCATGCACGA 223 5'-CCTGGCTCCTGCCTCCCA Gene Primer sequences Accession No.
A multicenter study performed in 477 patients with ALS (116 of whom had FALS) researched the genetic mutations in Turkey and found the following rates: C9orf72 (18%),
SOD1 (12.2%), FUS (5%), TARDBP (3.7%) and UBQLN2 (2.4%).
The toxic gain of function is the mechanism thought to lead to FALS caused by
SOD1 mutation.
Precipitated genomic DNA was amplified by real-time PCR with the following primers: Gapdh promoter region, 5'-ATCACTGCCACCCAGAAGACTGTGGA-3', and 5'-CTCATACCAGGAAATGAGCTTGACAAA-3';
Sod1 promoter region, 5'-AATAGCGACTTTCCCAGCTC-3', and 5'-AAACGAAGGTGCAAAACGAG-3'.
Patterson et al., "In vivo kinetic approach reveals slow
SOD1 turnover in the CNS," The Journal of Clinical Investigation, vol.
AST Increased the Protein Expression of HO-1 and
SOD1 in the Kidney of Diabetic Rats.
In the present study, we analyzed the activities of src and fyn tyrosine kinases and of protein tyrosine phosphatases (PTP), with particular interest to the striatal-enriched protein tyrosine phosphatase (STEP), in synaptosomal fractions prepared from the motor cortex and spinal cord of a transgenic mouse model of ALS (G93A mice), expressing wild-type human
SOD1 ([SOD1.sup.WT]) or overexpressing human mutant
SOD1 ([SOD1.sup.G93A]).
(a and b) Sophocarpine upregulated the expression of
SOD1, Nrf2, and CYPE2 and downregulated the levels of ROS protein detected by Western blot (a) and the semiquantitative analysis of lanes was based on optical density with ImageJ software (b); data are expressed as mean [+ or -] SEM, # P < 0.001.
In ALS, secreted
SOD1 is presented by MHC II by antigen-presenting cells, causing the activation and proliferation of CD4+ T cells (25).
A copper or zinc deficiency reduces the function and activity of the
SOD1 enzyme.