Rancidity, acidity index,
saponification number, density and humidity test were determined using the corresponding Mexican standard guidelines (NMX-F-222-SCFI-1995, NMX-F-075-SCFI-2006, NMX-F-174-SCFI-2006, NMX-F-211-SCFI-2006, NMX-F-101-SCFI-2012), while the peroxide number was determined in compliance with AOAC (2000).
Ismail, "Rapid and direct lodine value and
saponification number determination of fats and oils by attenuated total reflectance/fourier transform infrared spectroscopy," Journal of the American Oil Chemists Society, vol.
Saponification number was found to be 195 mg KOH/g oil and resembled that of honeydew melon (210 mg KOH/g oil) which was also a variety of Cucumis melo found by (Yanty et al., 2008).
This dried product contained hydroxylated oligomer and unreacted PET (low molecular weight) which were then separated by the procedure as described in our earlier study [19] and characterized for determination of
saponification number, hydroxyl value as per ASTM methods and for molecular weight by GPC technique.