The remaining aqueous extract was further fractionated into ethyl acetate fraction by using 500 ml ethyl acetate for three times in a
separatory funnel to obtain the solid residue (4.5 g).
The contents were poured through a funnel into a 100 mL
separatory funnel, along with 3 mL of 0.1 M NaCl in Milli-Q water.
After 30 min reaction to attain constant state, 200 mL solution is taken out from phenanthrene reaction system, followed by introduction into 400 mL
separatory funnel. 6g NaCl is added into the filtered solution.
After slowly inverting the
separatory funnel, two distinct layers were formed.
The obtained suspension was filtered by collecting the liquid into a
separatory funnel to separate the phases, and then the lipid extract was dried as reported earlier.
Details of the formulations used for unfunctionalized silica nanocomposites Formulation Factors F1 F2 [n.sub.HMDA]/ 3.0 3.3 [n.sub.ADCI] (a) VolumeTEOS (ml) 10 2.1 Reaction Time (sec) 90 120 Components added from HMDA & TEOS in water AdCl in toluene
separatory funnel to (aqueous phase) (organic phase) initiate reaction (a) The symbol n represents mole.
Flanagan notes that the way to check a phase separation in the laboratory is to dump the contents of a flask into a
separatory funnel, shake the funnel and wait for the phases to separate.
A total of 1.0 ml (density = 1.04 g/ml) of both plant essential oils were transferred to a 125 ml
separatory funnel and dissolved in 50 ml of hexane.
The water sample was transferred from the sample bottle to a 1000 mL
separatory funnel. Thirty mL of methylene chloride were added to the sample bottle, which was sealed and shaken for 30 seconds to rinse the inner surface.
The upper clear green ether phase was carefully decanted into a
separatory funnel and transferred to a pre-weighed round-bottom flask.