(18)-(20) AU-rich elements (AREs) and
stem-loop structures in 3' UTRs are well-studied examples of cis-elements recognized by a set of RBPs for controlling the stability of target mRNAs (Fig.
Stem-loops IIIa and IIIc were conserved among PBMCs and plasma-derived IRESes, except for a single mutation in
stem-loop IIIc at position 234 detected only in PBMC-derived samples (Figure 1).
As shown in Figure 1(a), at temperatures below 45[degrees] C, fluorescence intensity does not change with increasing temperature, which indicates that molecular beacon can maintain a stable
stem-loop structure.
We used a
stem-loop RT-PCR method to quantify the expression levels of various miRNAs.
In oocytes, some related factors are coupled to the SLBP, which contains the only RNA binding region and interaction with
stem-loop structure [26].
The OL region located between tRNA-Asn and tRNA-Cys comprised 35 bp in length within the WANCY region (Supplementary Table S1) and predictably had the capacity to fold into a stable
stem-loop hairpin structure consisting of 13 bp in the stem and 11 bp in the loop.
In the present study, we investigated the miR profiling in a small group of Chinese GC patients by applying a high throughput miR sequencing method and then validated the results by performing
stem-loop qRT-PCR in vivo and in vitro.
HV-GcV2 (2,262 nt) shares the same
stem-loop motif and genomic structure with HV-GcV1, with the exception of the rep gene, which is coded by a single open reading frame.
Comparison of the secondary structures of our isolated aptamers and aptamer A10 obtained by protein SELEX showed similar
stem-loop structures which could be responsible for the recognition of PSMA on LNCap cell surface.