Morphological changes, such as nuclear and chromatin condensation and fragmentation, that occur in apoptosis can be assessed by fluorescent microscopy using supravital dyes such as
acridine orange (AO) and ethidium bromide (EB).
Figure 2 shows that microbial counts in the soil column determined by
acridine orange direct counting (AODC) methods did not significantly change 1 week after flushing with ethanol compared with counts in soils before flushing (8.63 x 107 [+ or -] 2.22 x [10.sup.7] cells/g soil and 6.87 x [10.sup.7] [+ or -] 8.9l x [10.sup.6] cells/g soil, respectively).
Pattern of DNA denaturation was evaluated using fluorescent test of
acridine orange.
Acridine orange is intercalated into the nucleic acid of cells.
For analysis, two straws per replicate were thawed at 37AdegC, pooled and processed for
acridine orange staining.
Morphological observation of DAPI and
acridine orange/propodium iodide staining documented typical characteristics of apoptotic cell death.
Koehn, Dercitin, a new biologically active
acridine alkaloid from a deep water marine sponge, Dercitus sp., JACS, 110, 4856 (1988).
Sperm DNA integrity was assessed using
acridine orange assay (Martins et al.
Clearing allowed imaging with excellent cellular and nuclear resolution of SYTOX Green or
acridine orange-stained specimens more than 500 [micro]m deep into formalin-fixed human prostate, liver, breast, and kidney samples (Figures 1, a through d; 2, a through d).