Esta lectina permite reconocer terminales manosa, glucosa y glucosamina (Goldstein & Portez, 1986) de glicoconjugados localizados en la matriz
acrosomal, por lo que asociada a una fluoresceina puede identificar espermatozoides con el acrosoma intacto y diferenciarlos de aquellos que si han sufrido la reaccion acrosomica.
(28) The ultrastructure of sperms is reported to show that the PNA-binding site is situated on the outer
acrosomal membrane.
The presence of ROS in sperm samples produced an increase of permeability in plasmatic and
acrosomal membranes.
Acrosome reaction in abalone sperm involves the exocytosis of the
acrosomal vesicle and the polymerization of subacrosoreal materials, composed mainly of actin, to form the anterior spike called
acrosomal process (Neill & Vacquier 2004).
To induce the acrosome reaction, thereby exposing the inner
acrosomal components for lectin labeling, sperm were incubated with ionomycin calcium (Sigma Laboratories) to elevate intracellular calcium levels.
Efecto de la refrigeracion sobre la movilidad, integridad de membrana
acrosomal y reaccion
acrosomal en espermatozoides caninos.
Pattern C: sperm cells displaying fluorescence on the head that was greater in intensity at the
acrosomal zone, thus they were considered as undergoing
acrosomal reaction.
In vitro effects of l-carnitine and glutamine on motility,
acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage.
Evaluation of acrosome reaction and cell viability:
Acrosomal status was monitored with acrosome specific FITC-PSA (Lectin from Pisum sativum, L0770, Sigma Aldrich, Germany) which was dissolved in PBS.
El suplemento de linaza mostro una tendencia de reduccion de la resistencia de la membrana
acrosomal (Ort), asi como una tendencia de mejora de la integridad y funcionalidad de la membrana del espermatozoide (Host).