The colonies of Pestalotiopsis on PDA were whitish with crenated edges and
aerial mycelium. Acervuli formed after 20 days on PDA, whereas acervuli formed within 10 days when autoclaved carnation leaves were added to the PDA.
The colonies showed smooth borders and little
aerial mycelium to slightly cottony mycelium in the three culture media.
The morphology of the fungal clones that include peculiarities of the advancing (marginal) zone,
aerial mycelium, colony color, and possible changes in the reverse colony surface were observed and recorded.
4e) and broom like conidiophores on PDA borne from
aerial mycelium; stipes septate, apices nonvesiculate, smooth, thin walled, strictly monoverticillate (Fig.
The isolate produced cultures with a petaloid appearance, dense and cotton-like
aerial mycelium. The cardinal temperatures for growth of the isolate studied are in accordance with the descriptions of Erwin and Ribeiro (1996), for the P nicotianae species.
Micro- and macroscopic characteristics: the
aerial mycelium formed monopodially branched spore-bearing hyphae with the shape of loops, open or compact spirals with 3-6 curves (Figure 2).
ISP media
Aerial mycelium 1 Tryptone yeast extract agar (ISP 1) Nil 2 Yeast extract malt extract agar (ISP 2) Lavender to grey 3 Oat meal agar (ISP-3) Dark grey 4 Inorganic salt starch agar (ISP-4) Light grey 5 Glycerol asparagine agar (ISP 5) Grey 6 Tyrosine agar (ISP-7) Grey 7 Starch casein agar Grey S.
The JAJ18 had pink coloured
aerial mycelium and cherry red coloured substrate mycelium without diffusible pigments on modified ISP4.
Colonies were cottony in texture with fine white
aerial mycelium at surface.
Fungal colony was convex and pale greenish grey medium
aerial mycelium on surface.