Crystal structure of aminopeptidase N (proteobacteria
alanyl aminopeptidase) from Escherichia coli and conformational change of methionine 260 involved in substrate recognition.
DAP I by 429%, DAP II by 355% and DAP IV by 178%, followed by cathepsin L (246%), cathepsin D (123%), tripeptidyl aminopeptidase (109%), arginyl aminopeptidase (78%),
alanyl aminopeptidase (72%), leucyl aminopeptidase (49%), cathepsin H (37%) and proline endopeptidase (19%).
Similarly, the reaction of nitroso naphthol with tyrosine produced 1,2-Benzo-8-(
alanyl)-3-phenoxazone, (BLP) and was characterized by one and two-dimensional NMR spectroscopy and mass spectrometry (Bhansali & Kook 1993).
Alanyl aminopeptidase and dipeptidyl aminopeptidase IV activities were not disturbed.
Alanyl Glutamine--a stable dipeptide derivative of L-Alanine and L-Glutamine, which stimulates the synthesis of amino acids and proteins, enhances the immune system and also functions as an in vivo antioxidant.
Human proCNP 1-10 extended C-terminally with 4
alanyl and 1 tyrosyl residue was custom synthesized for tracer preparation and standards.
The cells expressed CD29 ([beta]1-integrin), CD13 (
alanyl aminopeptidase), CD44 (hyaluronate receptor), CD90.2 (thymocyte differentiation antigen-1b), and CD105 (endoglin, SH2).
However, synthetic [proBNP.sub.1-39] with the leucine zipper-like motif substituted with
alanyl residues also elutes as a molecule three times larger than the expected size, and the elution position can be reversed to the expected with use of denaturing conditions (Goetze et al., unpublished data).