The aliquotter can be pro- grammed for
aliquot volumes in secondary tubes ranging from 300 [micro]l to 9,999 [micro]l, processing up to 400 samples per hour while checking for clots.
It took ~20 min to perform triplicate measurements of all 3
aliquots from an individual patient.
The extension requires two additional steps to the traditional transformation protocol: to allow the students time to treat their DNA sample and then to have more plates available for the students to transform the appropriate number of
aliquots of competent cells (usually six: no DNA negative control, positive control of plasma with no treatment, amylase, protease, DNase, and RNase treatment).
Triple observations were performed for each
aliquot. Results were expressed as the cellular location in which fluorescence was observed for each experimental group: acrosome (A), equatorial band (E), postacrosome (P) and all over the head (H); and as a three-category ordinal scale for fluorescence intensity (low, medium and high intensity).
CONCLUSION: When comparing Charter Medical multiple
aliquot bag sets and the Charter Medical syringe
aliquot system to provide neonatal transfusions, the use of the syringe system decreased blood waste and proved more cost effective.
An
aliquot of VMS is used that has an amount of suppressor necessary to inhibit the deposition to the maximum possible extent, along with a corresponding amount of brightener (leveller intercept solution).
Duplicate
aliquots of each sample were treated with 10% trichloroacetic acid and sedimented at 1,000 x g for 15 min to precipitate macromolecules.
Rodby and Firanek (2003) compared the batch and
aliquot methods and decided that the
aliquot method was easier because there was less physical lifting, mixing and discarding of effluent.
John's wort were the most active single-entity herbal teas with
aliquots of extracts producing inhibitions >70% against three or more iso-form mediated-metabolisms (Table 4).
As the powder dissolves, a 20 mL
aliquot is withdrawn from the solution every 30 seconds for the duration of the 45-minute experiment.
This is prepared by injecting 2 mL of 0.9% saline into a 100-U vial of botulinum toxin, then drawing up a 0.4-mL
aliquot containing 20 U into a 1-mL insulin syringe.
Thus the measuring procedure had to be improved so that all the changes of sensitivity would be considered and only one
aliquot used (Duller 1995).