The Chromosomal changes observed (Figure 3) were mainly colchicine, disorganized metaphases, multipolar
anaphases and with loss of chromosomes.
Figure 1J-N shows the zygote at early
anaphase of the first meiosis viewed from the animal pole.
We found that Nesprin-2 was still associated with mitotic chromosomes and Nesprin-2 knockdown cells harbored increased numbers of chromatin bridges in
anaphase cells.
A: normal interphase, A1: binuclei at interphase, A2: micronucleus at interphase, B: normal prophase, B1: binuclei at prophase, B2: micronucleus at prophase, C: normal metaphase, C1: sticky metaphase, C2: C-mitosis, D: normal
anaphase, D1:
anaphase bridge, and D2: multipolar.
The process of mitosis is divided into five stages: prophase, prometaphase, metaphase,
anaphase, and telophase.
Cytokinesis is achieved by an actin-myosin contractile ring that physically divides the cell into two daughter cells following separation of the sister chromatids during
anaphase. Proper positioning of the contractile ring and, hence.
In addition, the frequency of chromosomal aberrations is increased, including chromosome bridges and laggard chromosomes and leads to partition-bundle division (a novel type of chromosomal aberration, in which chromosomes at the transition from
anaphase to telophase of mitosis are divided into three, four or six bundles) (Rong et al., 2002; Gao et al., 2010).
Together with the phosphorylation of serine 10 of histone 3, which is tightly associated with the condensation of chromatin until
anaphase of mitosis, this would be indicative for G2 arrest (Hendzel et al.