In univariate regression analysis (Table 2) between CC-resistant and CC-sensitive PCOS women, BMI, waist circumference, Ferriman-Gallwey score, oral glucose tolerance test (OGTT)- fasting/1 hr/ 2 hr values, HOMA-IR, LDL, HDL, triglyceride, cholesterol,
androstenedione, AMH, LH-FSH ratio, ovarian volume, and antral follicle count were found to be statistically significant.
Serum concentrations of pregnenolone, 17a hydroxyprogesterone, corticosterone, DHEA, and
androstenedione were measured by LC-MS method using an Agilent 1200 Series HPLC system for HPLC (Agilent Technologies Inc., California, USA) and an AB Sciex API5000 tandem mass spectrometer (AB Sciex Pte.
According to the study, "Salivary testosterone, cortisol, DHEA and
androstenedione levels did not change overall or between play against people vs.
Adrenal sex steroids, especially
androstenedione and to a lesser extent testosterone, are elevated in 21-OH deficiency owing to the accumulation of cortisol precursors that are diverted to sex hormone synthesis.
Androstenedione is considered a weak androgen in men and is secreted primarily by the testes.
The first one acts by converting pregnenolone in to 17OH-pregnenolone and progesterone into 17OH-progesterone; the 17,20-lyase activity converts 17OH-pregnenolone into dehydroepiandrosterone and, to a lesser extent, 17OH-progesterone into
androstenedione [15-17].
Supplementary text: detailed description of liquid chromatographytandem mass spectrometry method for serum total testosterone and
androstenedione. Supplementary Table 1: comparison of normal versus high total testosterone.
Following surgery, the patient did not require any analgesics, hormone levels significantly decreased as compared to before surgery with 17 hyroxyprogesterone levels of 972 nmol/lit and
androstenedione of 120 ng/dL, and her quality of life improved.
Hormones Patient's Normal range results Estradiol 645.32 * 55-165 pmol/L Cortisol level 50_550 nmol/L 8 AM 784.34 Midnight 453.00 17OH Progesterone 11.7 0.0-1.6 nmol/L
Androstenedione 8.82 0.3-3.1 ng/mL DHEA-S 1337 95-530 [micro]g/dL Total testosterone 6 10-40 nmol/L DHEA-S: dehydroepiandrosteronesulfate; * mean value.
In addition to glucocorticoids, to gain a better understanding of the effects of PFCs on steroidogenesis, we examined the association of PFCs with DHEA and
androstenedione as androgenic hormones in cord blood and assessed the balance of glucocorticoids and androgenic hormones in infants.