The plates were then developed with toluene:ethyl acetate, 93:7, as a mobile phase and another TLC plate for
bioautography was prepared in parallel.
Bioautography determines the presence of compounds in the extracts with antioxidant activity.
The results of
bioautography show the antibacterial activity in different bio-compounds (Figure 2).
TLC
bioautography (TLC bio) is an effective and inexpensive technique that combines the chromatographic separation with in situ localization of compounds with biological activity [30-32].
Those aspects constitute Schneemanns version of what I call
bioautography. This critical neologism inverts the normative term "autobiography": instead of the "self-life-writing" order of the term autobiography, the term
bioautography gives primacy to the bio-life in self-writing.
The qualitative test (
bioautography antioxidant assay) involved DPPH only, and the quantitative test involved DPPH, hydrogen peroxide, and reducing assays.
Extraction methods and
bioautography for evaluation of medicinal plant antimicrobial activity.
Inayat, Antibacterial Activities of Dodonaea viscose using Contact
Bioautography Technique.
The compounds contained in these extracts over silica gel were also evaluated through
bioautography.