We also evaluated the peripheral blood
blast cell percentage as well as bone marrow
blast cell percentage.
The blast counts on the first presentation of ALL patients showed that majority of ALL cases, 21 cases (56.76%) showed
blast cells between 50 to 80% followed by 8 cases (21.63%) showed more than 80%
blast cells.
Clinical and laboratory characteristics at diagnosis were statistically correlated (age, sex, WBC count, hemoglobin level, PLT count,
blast cells rate) with t(5;17), t(8;21), inv(16) chromosomal aberrations, and FLT3 mutations (Table 1).
In this study, univariate analysis showed that T-ALL, high LDH at diagnosis, age ≥35 years,
blast cell proportion ≥5% on the 15 [sup]th day of induction therapy, and extramedullary infiltration before HSCT were the poor prognosis factors.
To support the new freezer and
blast cell operations, the low temperature distribution and food processing facility has an upgraded machine and mechanical room.
It is based on number of
blast cells and partially or fully differentiated mature cell type on smear of the blood and/or bone marrow sample.
60% of the patients having
blast cell less than 20% and 66.66% having
blast cell more than 20% have no retinopathy.
As a matter of fact, in a more recent study (140) on a series of 111 AML patients in which multivariate information provided by the use of multiple stainings analyzed at flow cytometry was obtained, it has been shown that the combination of three phenotypic variables (the number of major
blast cell populations, the pattern of CD34/CD15 expression, and the reactivity for CD13) was highly sensitive (100%) and specific (99%)for the selection of AML cases carrying PML/RAR-[alpha] gene rearrangements as demonstrated by the combined use of the reverse transcription-PCR and fluorescence in situ hybridization.
The
blast cell suspensions were thawed at room temperature.
Combined evaluation of the Blast flag with the Abn Lympho/L_Blasts flag on the XE-2100 did not increase the sensitivity of
blast cell flagging but resulted in an increase of FP results.