Muscle samples were washed in 0.1 M sodium
cacodylate buffer, post-fixed in 1% Os[O.sub.4] for 2 h at room temperature, washed again in the same buffer followed by distilled water, contrasted with 5% aqueous uranyl acetate solution for 1 h and finally washed in distilled water (Rocha, Leonardo, De Souza, Palma, Da Cruz-Hofling, 2008; Rocha, Souza, Palma, da Cruz-Hofling, & Harris, 2009).
Under anesthesia (see above), the rats were perfused with 4% formaldehyde in 0.1 M
cacodylate buffer, pH7.3, for 15 min at room temperature (RT).
Then, the segments were fixed in a buffer containing 2% glutaraldehyde and 0.1 M
cacodylate for 30 min followed by rinsing three times for 10 min in 0.2 M
cacodylate buffer.
Later glutaraldehyde was drained and rinsed 3 times, each time for 5 minutes, in sodium
cacodylate solution buffer.
Decellularized diaphragm specimens were fixed in 2.5% glutaraldehyde (Merck, USA) with 0.1M
cacodylate buffer (Prolabo, Sweden) at room temperature for 2 h.
For scanning electron microscopy (SEM), 2 parasites were washed five times with 0.2 M
cacodylate buffer (pH 7.3), fixed in 2.5% glutaraldehyde and post fixed in 1% osmium tetroxide at 4degC.
L'Oeil
cacodylate (1921) is a large graffiti collage of signatures, doodles, and sayings of Picabia's circle of friends, lovers, collaborators, and French celebrities.
Marinated and control samples (10 mm x 2 mm x 2 mm) were soaked for 24 h at 4[degrees]C in a 0.2 M glutaraldehyde solution with 0.2 M sodium
cacodylate buffer (pH 7.4), rinsed 5 times in 0.2 M sodium
cacodylate buffer, and fixed in 4% osmium tetroxide in 0.2 M sodium
cacodylate buffer for 2 h at 4[degrees]C.
The specimens were prefixed in 2.5% glutaraldehyde in
cacodylate buffer at 4[degrees]C during the night.
Samples containing [10.sup.6] erythrocytes/mL were left still for 30min, followed by fixation in Karnovsky's fixative (2.5% glutaraldehyde and 4% paraformaldehyde in sodium
cacodylate buffer 0.1 M, pH 7.4) dehydrated in alcohol and stored on a glass surface for 16 h at 4[degrees]C to be prepared for observation under a scanning microscope (JSM 6390 LV Low Vacuum; JEOL, Peabody, MA, USA).