(b) Samples were incubated with increasing concentrations of eculizumab (0-100 [micro]g/mL) and
classical pathway complement activation was measured by the Wieslab[R] assay, detected as % of a normal human standard sera by the kit, defined to contain 100% activity (% of NHS).
Jass' subtypes 1 and 2 and subtype 4 reflect CRCs arising from the serrated neoplasia pathway and the
classical pathway, respectively.
Hemolytic activity in
classical pathway and alternative pathway was associated with variation of the porcine genes encoding the complement components pC3 (Wimmers et al., 2003), the pC5 (Kumar et al., 2004), and the porcine mannose-binding lectin 1 (pMBL1) and pMBL2 (Phatsara et al., 2007).
As we know, the PI3-K/AKT signal pathway is a
classical pathway of insulin in glucose metabolism, having a role in glucose uptake by the liver, skeletal muscles, and adipose tissues [19, 20].
Then, CRP is able to interact with C1q, activating the
classical pathway of the complement system [49].
C1q is the first component in the
classical pathway of complement activation.
In the
classical pathway UROGEN is then converted into heme via the intermediates coproporphyrinogen III, protoporphyrinogen IX, and protoporphyrin IX [2].
The
classical pathway is activated by an antibody bound to a foreign particle.