Another method introduced for quantification of amikacin, kanamycin, streptomycin and neomycin described the use of cyanoacetamide as derivatizing agent for their UV measurement [11].
Zakhari, Spectrophotometric assay of certain aminoglycosides using cyanoacetamide, Anal.
Summary: Various cyanoacetamide based Knoevenagel adducts were coupled with barbituric acid / thiobarbituric acid and triethylorthoformate via a one pot three component reaction in 2-butanol availing the desired compound in excellent yields.
Similarly cyanoacetamide derivatives are known to be useful as herbicidals and plant growth regulators with biological and pharmaceutical interest [13-15].
All the Knoevenagel adducts were availed in good to excellent yields when an aldehyde (0.02 moles) and cyanoacetamide (0.02 mole) was charged into a round bottom flask having 15 mL of dry ethanol.
Scheme-2: Synthesis of Cyanoacetamide based barbiturates/thiobarbiturates.
Cyanoacetamide based Compounds (2-15) were synthesized in good to excellent yields (5987%) in solid state from hot stirred solution of (1a1o) and barbituric/thiobarbituric acid in triethyleorthoformate and 2-butanol after refluxing for 4-5 hours.
The 1H-NMR spectra of cyanoacetamide based compounds (2-15) displayed proton signals in the appropriate regions.