dark-field illumination
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dark-field illumination
[′därk ‚fēld ə‚lüm·ə′nā·shən] (optics)
A method of microscope illumination in which the illuminating beam is a hollow cone of light formed by an opaque stop at the center of the condenser large enough to prevent direct light from entering the objective; the specimen is placed at the concentration of the light cone, and is seen with light scattered or diffracted by it.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive
Dark-field illumination results in most of the light falling outside of a camera's field of view (FOV).
It has been suggested that Leeuwenhoek might have used some simple means of
dark-field illumination to visualize details such as flagella on bacteria.
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