Estrous behavior, ovulatory follicle dynamics, and corpus luteum size in Creole cows after spontaneous or prostaglandin F2[alpha]-induced
estrous.
Table I.- The time of
estrous cycle in different seasons in Dazu black goat.
The reproductive tracts of gilts were obtained immediately after slaughter at a local slaughterhouse on either days 0, 3, 6, 9, 12, 15, or 18 of the
estrous cycle or days 12, 15, 30, 60, 90, or 114 of pregnancy (n = 3-6/d/status).
The staining of vaginal smears with Panotico or Giemsa has been reported as a way to identify the different phases of the
estrous cycle in ewes (Porto et al., 2007; Zohara et al., 2014).
Determination of estrus cycle stage: Since menstrual disturbance is a typical clinical feature of PCOS women,
estrous cycles were examined in all groups of mice by evaluating the cell types in vaginal smears.
In addition, OVX animals treated with different doses of TMX were stable at the estrus phase of the
estrous cycle.
There was only one
estrous HD female in the test, which complicated the statistical analysis of the
estrous cycle.
Vaginal smears from 70 days of age show that taiep rats have irregular
estrous cycles when compared to SD rats (Figure 6).
Separately, an effect of age on
estrous repetition was observed (P<0.001), since that problems was more common (odds ratio: 1.84; 95% CI: 1.04 to 3.25) in animals older than 6 years (42.3%) when compared to animals with 1-3 years old (27.9%).
Reproductive condition was established only in adult females by observing external morphological characteristics such as nipples development and the state of the vagina (closed, open or vaginal plug), and the analysis of samples of vaginal swabs for determining the phases of the
estrous cycle.
Animals, samples and determination of
estrous cycle.
Starting the Ovsynch protocol on 6th day of
estrous cycle improves pregnancy rates compared with use of Ovsynch protocol on random stages of
estrous cycle due to greater ovulatory response to first GnRH treatment and because of ovulatory follicle coming from the new wave of follicular development induced by first GnRH treatment of Ovsynch protocol.