The polymerase often binds to a promoter found near the DNA sequence encoding what will become the
hairpin loop of the pre-miRNA.
The most prominent problem associated is
hairpin loop, which directly interferes during annealing of primers on difficult DNA template that leads to no amplification.
Typically, tails are added to the primer that is closer to the variation to form the smaller
hairpin loop. Smaller loops minimize the chance of additional secondary structure and increase hairpin [T.sub.m]s so that shorter stems can be used (16).
In contrast, the coupled state exhibits repetitive patterns of
hairpin loop formation that are likely to be force-generating (Fig.
- a stuffer fragment (which is a length of double stranded DNA coding for a
hairpin loop in the dsRNA.
(3,6,9,10,11) The single-stranded oligonucleotide probe however contains complementary sequences at the end, which bind and form a
hairpin loop structure.
Different oligonucleotides with inverted repeats were designed to form
hairpin loop structures to test efficiency of the sodium bisulfite reaction method.
Which molecular probes contain complementary sequences at the end forming a
hairpin loop structure and use FRET technology to measure the intensity of the fluorescent signal generated?
A snapback primer combines a standard primer with an oligonucleotide tail that "snaps back" to hybridize with its extension product, creating a
hairpin loop in the single-stranded product.
I did not come to Norway looking for you, but you are everywhere, your A Minor Concerto pulsating with the
hairpin loops of our bus high above the fiords.