homogenate

[hə′mäj·ə·nət]

(biology)

A tissue that has been finely divided and mixed.

The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Homogenate

a suspension of subcellular particles of pulverized animal or plant tissue in a buffer or other solution; the suspension is usually obtained with the aid of a special device called a homogenizer. Differential centrifugation, among other methods, is used to separate such microscopic cell structures as organelles and such molecular structures as proteins, nucleic acids, and lipids from the homogenate. Homogenates are also used to study biochemical reactions outside an organism (in vitro).

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References in periodicals archive

Assessment of Catalase (CAT): Tissue homogenate (0.1 ml) was mixed with phosphate buffer (1 ml) and hydrogen peroxide (0.4 ml) and later on mixed with dichromate acetic acid reagent (2 ml).

MEMORY ENHANCING EFFECT OF ANISE (PIMPINELLA ANISUM) WITH RESPECT TO ITS ANTIOXIDANT ACTIVITY IN ALBINO MICE

In the brain homogenates, the levels showed an increase in the RC (P<0.001) and DR (P<0.001) groups.

Radioprotective Efficacy of Lutein in Ameliorating Electron Beam Radiation-induced Oxidative Injury in Swiss Albino Mice

Homogenate extraction was carried out on JHBE-50A homogenate extractor (Golden Star Technology, Inc., Ltd., Zhengzhou, China).

Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

As noted, a significant increase in the amount of this protein was detected after 3 and 4 hours of perfusion in homogenates of these hearts.

Leptin Modifies the Rat Heart Performance Associated with Mitochondrial Dysfunction Independently of Its Prohypertrophic Effects

In terms of spatial learning performance in Morris water maze test, matching the results from assays of sialic acid levels, the cubs whose female parental generation individuals were orally administered high-dose EBN homogenate during pregnancy or both pregnancy and lactation periods exhibited better memory function and level of intelligence.

Effect of Maternal Administration of Edible Bird's Nest on the Learning and Memory Abilities of Suckling Offspring in Mice

Then the homogenates were centrifuged at 4[degrees]C for 20 min at 15,000xg, the supernatant was collected for sarcoplasmic protein analysis.

Effects of dietary energy sources on early postmortem muscle metabolism of finishing pigs

Property PMCA RT-QuIC Prion disease vCJD Sporadic, familial, specificity and acquired [PrP.sup.C] Transgenic mouse Truncated recombinant substrate brain homogenate hamster [PrP.sup.C] expressing human (2nd generation assays) [PrP.sup.C] Fragmentation Sonication Shaking technique Fluids and tissues Blood, urine CSF, nasal brushings tested Detection Western blot Thioflavin T fluorescence Turnaround time [congruent to]11 days <24 h Currently in No Yes clinical use

Amplification of Misfolded Prion Proteins in Blood and Cerebrospinal Fluid for Detection of Creutzfeldt-Jakob Disease

Application of the method to rat brain tissue homogenate

OPTIMIZATION OF ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) WITH FLUORESCENCE DETECTOR (FLD) METHOD FOR THE QUANTITATIVE DETERMINATION OF SELECTED NEUROTRANSMITTERS IN RAT BRAIN

To investigate the potential relative therapeutic effects of Crataegus aronia and simvastatin on upregulation the antioxidant GSH and suppression the oxidative stress TBARS and lipidemia, we measured reduced GSH and TBARS biomarkers in liver homogenates and lipid fractions (cholesterol, triaceylglycerol, and low density lipoprotein (LDL)) in the blood of rats sacrificed after 15 weeks (Table I).

Differential Therapeutic Effects of Crataegus aronia and Simvastatin on the Hepatocyte Ultrastructure in Hepatic Steatosis/ Efectos Terapeuticos Diferenciales de Crataegus aronia y Simvastatina sobre la Estructura del Hepatocito en la Esteatosis Hepatica

To determine the relative sensitivity of PMCA, we retitrated a reference sample (10% cerebral cortex homogenate from a vCJD-affected patient) that had previously undergone endpoint titration (IC inoculation route; Table 1) in bovine PrP-expressing mice (tgBov).

Distribution and quantitative estimates of variant Creutzfeldt-Jakob disease prions in tissues of clinical and asymptomatic patients

[18] After analyzing the test samples, the remaining homogenates were mixed and then equal volume of homogenate mixture was added to different standard concentrations.

Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models