Thus, ATP was degraded into xanthine or
hypoxanthine via IMP or inosine generation, and GTP was degraded into GMP and guanosine in parallel during the aging process, resulting in increased contents of uric acid and PRPP.
Maximum values of
hypoxanthine did not exceed 1.06 [micro]mol [g.sup.-1] in the 336th hour of storage, for the intensity in 150 volts.
iodinea cerata and body epithelium were analyzed by TLC, in parallel with pure guanine and
hypoxanthine (Fig.
Dipotassium hydrogen orthophosphate, potassium dihydrogen phosphate, creatinine, uric acid,
hypoxanthine, and sodium hydroxide were purchased from Riedel-de Haen AG, Seelze, Hannover.
Hypoxanthine, aminopterin, and thymidine (HAT) were used to culture NG108-15 cells; however, aminopterin in HAT was toxic to INS-1 cells (Figure 2(a)).
Analysis of correlation matrix showed high correlation of BCAAs and cysteine, PPP metabolites (except ribose 5-phosphate), and inosine and its metabolites (
hypoxanthine and xanthine).
In vitro analysis is comprised of microscopy-based assay (also known as WHO microtest), tritium-labeled
hypoxanthine assay (isotopic assays), fluorimetric assay (using DNA-specific dyes), cytometry-based assay, and enzyme-linked immunosorbent assay (ELISA) [31, 32].
The levels of cholesterol, myo-inositol-1-phosphate, uracil, and
hypoxanthine were higher in both serum and tissues of the EC patients than those of the controls.
Among other metabolites detected in AH (Table 6) several molecules with antioxidative properties (e.g., citric acid, ascorbic acid, quinic acid, pantothenic acid, betaine, or taurine) or related to oxidative stress (e.g., pyroglutamic acid or
hypoxanthine) were identified.