Lecithinase or PC-PLC activity was absent in all isolates under the conditions in which this was determined.
(1968) Studies on the
lecithinase from Penicillium notatum.
The phenotypic determination of possible virulence factors showed that the isolates (VP1-VP3 and VR1-VR3) all produced [beta]-hemolysis on rabbit blood agar,
lecithinase, proteinase, gelatinase, and DNase.
botulinum was identified and differentiated from other botulinum toxin-producing clostridia on the basis of Gram stain reaction, morphologic features, motility, lipase and
lecithinase reactions on egg yolk agar, sugar fermentations, esculin hydrolyis, gelatin liquefaction, and production of botulinum neurotoxin.
The organisms also grew on chocolate agar and were positive for catalase and
lecithinase. The organism was susceptible to vancomycin and levofloxacin and was resistant to ampicillin and ceftriaxone.
Biochemical and morphological characterization: The microbiological identification of the isolates was done by: Gram,
lecithinase and haemolytic activity, screening for presence of parasporal bodies by phase-contrast microscopy.
The S.epidermidis species with various prevalence showed such signs as flocculation factor, fermentation of maltose, lactose and mannite,
lecithinase and haemolytiic activity.
* Enzyme activity: Amylase, Protease, (Caseinase, Gelatinase), lipase,
Lecithinase, & Tyrosinase
cereus colonies showing
lecithinase reaction were picked up, purified and stored on nutrient agar slant for further confirmation, which was carried out by Gram's staining and standard biochemical reactions (14).
mallei produce toxins such as pyocyanin (blue-green pigment that interferes with energy production via the electron transfer system),
lecithinase (causes cell lysis by degrading lecithin of cell membranes), collagenase, lipase, and hemolysin.