We presume such a strategy may be employed in setting up a tooth bank as reported elsewhere.14 The explant method has been extensively used for studying the dental pulp cells physiology, cell subpopulations capable of differentiating into
odontoblasts or mineral-forming cells in vitro15 and dentin-like structure in vitro.16 DPSCs and SHED obtained from enzyme digestion were also capable of differentiating into odontoblast-like cells and produced dentin in vivo.6,7 Furthermore, an immature DPSC (iDPSC) population was obtained from SHED via explant method.
However, dental caries were verified in diabetic group; revealed irregular pulp tissue and the dilation of blood vessels besides hemorrhage and a significant increase in inflammatory cells hyperplasia in
odontoblast cells.
Odontoblast markers were evaluated by immunohistochemistry at 7,14, and 21 days after reseeding in fresh and thawed EXP-21 cells.
Autoradiographic analysis of
odontoblast replacement following pulp exposure in primate teeth.
Furthermore, NO expression in
odontoblasts during tertiary dentinogenesis is synchronized with other
odontoblast differentiation markers, such as osteocalcin and alkaline phosphatase (Mei et al., 2007).
Our data showed that ginsenoside Rg1 significantly stimulated DPSCs proliferation and promoted the
odontoblast differentiation.
NGF was also characterized as a potent promoter of mineralization during dentin formation and it also promotes in vitro
odontoblast differentiation (26).
Lefeuvre et al., "Establishment of odontoblastic cells, which indicate
odontoblast features both in vivo and in vitro," Journal of Oral Pathology and Medicine, vol.
Odontoblast RNA stability in different temperature-based protocols for tooth storage.
Bmp4 is important during the bud-cap stage transition, as it activates Msx1 and Msx2 in the mesenchyme and is necessary for molar development and
odontoblast differentiation [Nie et al., 2006b].