Joe River and since the quality of their
polytene chromosomes was excellent, I compared chromosome diversity within and among samples taken in 2011, 2012 and 2013 (avg.
It was not possible for us to utilize the first and second instar larval stages for species identification, as they are small, present deep inside the food and their
polytene chromosome preparations are not very good.
Mitotic karyotype analysis and
polytene chromosome correlations.
Polytene chromosomes and phylogenetic relationships of Chironomus atrella (Diptera: Chironomidae) in North America.
We used conventional methods of collection (Shields and Procunier, 1982) and identification of larvae (Currie, 1986), the Feulgen method of Rothfels and Dunbar (1953) to stain
polytene chromosomes and gonads of larvae and the standard chromosome maps for the Simulium arcticum complex (Shields and Procunier, 1982) to determine chromosomal variation.
The genotypes of adult females and their mates are determined by cytological examination of
polytene chromosomes from 12 offspring.
1979), chromosome pairing in interspecific hybrids (Throckmorton 1982; Krimbas and Loukas 1984), banding patterns in
polytene chromosomes (Ashburner and Berendes 1978), and in situ hybridization (Whiting et al.
Immunological method for mapping genes on Drosophila
polytene chromosomes. Proc Natl Acad Sci USA.
For example, he concluded that
polytene chromosomes of insects' salivary glands and other tissues represented a particular form of endopolyploidy and suggested that there were probably different levels of replication of the chromosome thread in different regions of some
polytene chromosomes.
For example, both chapters on ISH to
polytene chromosomes are nearly the same as in the first edition, down to the illustrations and references, and both cover similar material.