Score 2 is characterized by Sertoli cells only, scoring 3 by spermatogonium, scoring 4 is characterized by low amounts of
primary spermatocytes, whereas scoring 5 is dominated by
primary spermatocytes.
Quantification of Germ Cells at Stages VII and VIII in Seminiferous Epithelial Cycle: The H&E stained slides were examined under a light microscope (Zeiss/German) and different cells (sustentacular or sertoli, Spermatogonia [Sg],
Primary Spermatocytes [Sc], Spermatid and Spermatozoid) present at this stage were counted with an objective lens x40.
or testicular biopsy,
primary spermatocyte testicular stage.
The second type of spermatogonia gives rise to
primary spermatocytes. In this aspect, M.
Based on cell size and nuclear characteristics, the present study revealed 10 stages of spermatogenic cells: spermatogonia, 5 stages of
primary spermatocyte, secondary spermatocyte, 2 stages of spermatid, and spermatozoa.
The "ripe" stage in triploids, which represented the observed culmination in gonad development for triploids in this study, consisted of gonads with mostly
primary spermatocytes in males and typically few oocytes in females.
The spermatogonia and
primary spermatocytes detected in the STs, which contained MNGCs, expressed more bax protein when compared with other cells.
The presence of
primary spermatocytes at two different stages, specifically zygotene and diplotene, with large nuclei observed in spermatocytes in the diplotene stage, was the other notable characteristic of this stage.
Tubules with continuous germinal epithelium, and discontinuous (bar= 5 [micro]m): lumen (*), connective tisuue (tc), interstitial tissue (**), spermatogonia (ep), spermatozoa (ez), tunica albuginea (ta), principal tubules (pt),
primary spermatocytes (esp), secundary espermatocysts (es), spermatids (et), Leydig cells (cl), Sertoli cells (cs).
Primary spermatocytes, stained with the anti-SCP3 antibody, were selected to evaluate the respective percentages of leptotene, zygotene, pachytene and diplotene stages.
As it was noticed in this study, a clear reduction in the testis content for each of the spermatocytes in general, Spermatogonia cells and especially
primary spermatocytes. This result is consistent with what was indicated by other studies about that the treatment by one of anti-cancer drugs such as Cyclophosphamide has caused a shortage in the number of Spermatogonia cells and
primary spermatocytes in male rats [23].