Alternatively, low expression of miR-200c in the
primary tissue might reflect changes taking place in the primary tumor associated with EMT that facilitate exit from the primary tumor, a process that is later reversed in the metastatic deposit.
Briefly, 2 x [10.sup.5] or 5 x [10.sup.5] cells (for RNA or protein extraction, respectively) were plated onto six-well plates or 60 mm
primary tissue culture dishes (Becton Dickinson Labware) and transfected with plasmid DNA using the X-tremeGENE HP DNA transfection reagent[R] (Roche Diagnostics GmbH, Germany), in the proportion of 1 [micro]g plasmid DNA and 1 [micro]L transfection reagent per 1 x [10.sup.5] cells.
In the current study, fibroblastlike cells were found to grow and expand from the periphery of the
primary tissue explants.
Shoot and root meristems (see Figure 7-8) that cause a plant to grow vertically (higher in the air and deeper in the ground) comprise the apical meristems, giving rise to the
primary tissue of a plant.
This collection of articles covers intelligent biomatrices and engineered tissue constructs, bioreactor design for three-dimensional tissue culture, bioelectronic and biosensoric microstructures supporting high-content screening, techniques for toxicity testing and biomonitoring of airborne contaminants, drug screening using cell lines, cell lines and
primary tissues for in-vitro evaluation of vaccines, designer cells derived from
primary tissue and designed cell lines as a sustainable cell source, human embryonic stem cell research and its impact on in-vitro drug screening technologies, availability and safety of human cells and tissues, the ethics and scientific rationale for in-vitro alternative to animal testing, and the benefits of further research.
This article describes how to create a
primary tissue culture of chick embryo cardiac cells.
Scott Paper is the
primary tissue producer in Canada with close to 40% market share.
The company may have carved out a niche in the database market through its use of
primary tissue samples for gene expression analysis, which no other public or private database offers.
"It is very difficult to get
primary tissue to study until after a patient has died," said the Royal Edinburgh Hospital's Professor Andrew McIntosh, who is collaborating with the center on the project.
The cutoff (for 95% specificity) derived from the data on metastatic tissue was slightly higher than that for
primary tissue data, but the distributions of serum HER2 in the negative (
primary tissue vs metastatic tissue) and positive tissue HER2 group showed no significant difference by t-test (P = 0.56 in tissue HER2 negative group, and P = 0.63 in tissue HER2 positive group).