Cytoplasmic vacuolation with several degenerated areas, damaged mitochondria,
pyknotic nuclei, increased number of lysosomes and cellular debris in the lumen were observed.
Histopathological scoring Groups AA E EA V PN MC Healthy 0 0 0 0 0 0 AR 3 2 3 0 3 3 AR+desloratadine 2 1 2 0 2 2 AR+misoprostol 1 0 1 1 2 0 AR+desloratadine+misoprostol 0 0 1 1 1 0 E: edema; AA: acinar atrophy; EA: inflammation area; V: vacuolization; PN:
pyknotic nucleus; MC: mast cell.
Prominent microscopic lesions in lymphoid organs included intra cytoplasmic inclusion bodies, syncytial cell formation, depletion and necrosis of lymphoid tissues with numerous lymphocyte infiltration with
pyknotic nuclei, seen from day 14 to 21 of infection in untreated group B (Plate 3).
Dying cells were initially identified in sections stained with H&E, as having a shrunken
pyknotic nucleus set in an intensely eosinophilic cytoplasm.
The goblet cells appear to be clustered and clotted in mucine clusters associated with the presence of metaplasic cellular specimens with some
pyknotic nuclei.
Caption: Figure 4: Histological changes of the liver: (blue arrow) hepatocytes with hydropic degeneration; (red arrow) hepatocytes with hyaline degeneration (Mallory corpuscles); (black arrow)
pyknotic nuclei.
Features of apoptosis include a
pyknotic nucleus that dyes brown with uneven coloring and is typically ring shaped.
Degenerative changes include cytoplasmic rupture, loss of nuclear shape, elongation or indistinct segmentation of granulocyte nuclei, loss of chromatin definition (appear to have liquefied),
pyknotic nuclei (necrobiotic forms), degranulation and cytoplasmic vacuolation.
Distinguishing
pyknotic cells or degenerating cells from true mitotic figures can be challenging.