Raffinose

The best-known trisaccharide (oligosaccharide), widely distributed in higher plants. The best-known sources are cottonseed meal and the manna of Eucalyptus. It is also known as melitose, melitriose, gossypose, and O-α- d -galactopyranosyl-(1→6)-O-α- d -glucopyranosyl-(1→2)-β- d -fructofuranoside. See Oligosaccharide

Complete acid hydrolysis gives 1 mole each of d -galactose, d -glucose, and d -fructose. In structure, it comprises melibiose and sucrose with the central d -glucose in common. See Fructose, Galactose, Glucose

Raffinose can be hydrolyzed by enzymes in two ways. Invertase (β- d -fructofuranoside) hydrolyzes the sucrose part of the molecule to give melibiose and d -fructose. Almond emulsin, which contains an α- d -galactosidase, hydrolyzes the melibiose residue to yield d -galactose and sucrose.

Raffinose was found to be enzymically synthesized in plants from uridine diphosphate d -galactose and sucrose by an enzyme which transfers the d -galactose moiety of this sugar nucleotide to sucrose, resulting in the formation of raffinose.

raffinose

[′raf·ə‚nōs]

(biochemistry)

C₁₈H₃₂O₁₆·5H₂O A white, crystalline trisaccharide found in sugarbeets, cottonseed meal, and molasses; yields glucose, fructose, and galactose on complete hydrolysis. Also known as gossypose; melitose; melitriose.

The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Raffinose

a nonreducing trisaccharide composed of residues of D-galactose, D-glucose, and D-fructose. Raffinose is a colorless water-soluble substance with melting points of 80°C (pentahydrate) and 119°–120°C (anhydrous). It is one of the most widely distributed stored carbohydrates in plants (sugar beet, cottonseed meal, Australian manna). The enzyme a-galactosidase splits raffinose into galactose and the disacchar-ide saccharose, and invertase splits it into fructose and melibiose.

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References in periodicals archive

L40 strains expressing indicated pCL313-Med6 derivatives were grown in synthetic media containing glucose (Glc) or galactose plus raffinose (Gal + Raf) for 6h in the presence of copper.

Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex

The sucrose and raffinose content was the best predictor for AID and SID of N, lysine, threonine, and valine.

Oligosaccharides are a key factor in prediction of amino acid digestibility in soybean meal of different origins when fed to growing pigs

The numbers of wasps tested on each sugar--concentration treatment were as follows: sucrose (0.1 M: n = 14; 0.5 M: n = 21; 1.0 M: n = 36); melizitose (0.1 M: n = 6; 0.5 M: n = 14; 1.0 M: n = 19); raffinose (0.1 M: n = 5; 0.5 M: n = 13; 1.0 M: n = 10); glucose (0.1 M: n = 6; 0.5 M: n = 10; 1.0 M: n = 19); fructose (0.1 M: n = 3; 0.5 M: n = 10; 1.0 M: n = 19); and maltose (0.1 M: n = 6; 0.5 M: n = 8; 1.0 M: n = 7).

Laboratory evaluations of the foraging success of Tamarixia radiata (Hymenoptera: Eulophidae) on flowers and extrafloral nectaries: potential use of nectar plants for conservation biological control of Asian citrus psyllid (Hemiptera: Liviidae)

For raffinose synthase (MU-RS2-1 and MU-RS2-2) assays, mutant sources (PI 200508 and mutagenized line 397) and artificial heterozygotes were tested along with a wild-type genotype, cultivar Williams 82.

Development of SNP Genotyping Assays for Seed Composition Traits in Soybean

mesenteroides Strain Carbohydrates V1 Arabinose + Glucose + Galactose + Lactose + Fructose + Saccharose + Sorbitol - Mannitol - Maltose + Rhamnose - Raffinose - Xylose - V1: Leuconostoc mesonteroides subsp mesonteroides Table 3:Effect of pH on the viability of the strain Leuconostoc pH2 pH3 pH4 0h 3h 0h 3h 0h 3h 5,13 0 7.45 7.67 7.14 7.77 These results are expressed as log CFUN.

Evaluation of technological properties of Leuconostoc mesonteroides (V1) strain isolated from Algerian goat's milk

They are capable to ferment sugars including glucose, lactose, maltose, galactose, fructose, sucrose, starch, and raffinose due to the specific enzymes produced by test species during sugar fermentation (Table 2).

Epidemiological Studies of Potent Environmental Pathogen: Streptococcus pneumoniae

(38,39) Garbanzo beans contain a significant amount of indigestible resistant starch and oligosaccharides such as raffinose that work in tandem to restore a healthy and balanced gut microbiome.

Garbanzo beans

Both germs are negative for catalase, arabinose, and raffinose while being positive for maltose, fructose, and mannitol [4, 5].

First Case Report of a Late Onset Knee Periprosthetic Joint Infection Caused by Lactococcus garvieae

The activity was inhibited to a lesser degree by disaccharides and trisaccharides like lactose, melibiose and raffinose, all of which are saccharides with a terminal non-reducing galactose (Table 1).

Purification and partial characterization of a ribosome-inactivating protein from the latex of Euphorbia trigona Miller with cytotoxic activity toward human cancer cell lines