As shown in figure 3,
recombinant protein was expressed and accumulated in the insoluble fraction.
Also, identification of the produced
recombinant protein was specifically confirmed by SDS-PAGE and western blotting.
Recombinant Protein A conjugated with HiLyte[TM] Fluor 488 can be used for immunoglobulin detection in IHC and IF and for assay development.
The addition of Primorigen's Vitronectin XF and platform
recombinant protein production technology will play a huge role in Nucleus' strategy of offering traceable, reproducible and xeno-free cell culture ecosystems for the cell and gene therapy space.
Starting from a small supplier of proteins and enzymes for academic institutes and biotech companies, Creative BioMart has always been focusing on developing high quality protein products including
recombinant protein, GMP proteins, native proteins, assay kit, etc.
Recombinant protein expression in a Drosophila cell line: comparison with the baculovirus system.
In addition, Grifols recently began an engineering project to convert the 9,000 square foot expansion space within CMF into cell culture and purification suites for production of
recombinant proteins expressed in mammalian cells.
The company's new USD80m Consolidated Manufacturing Facility supports development and manufacturing services to third parties for
recombinant protein production.
Expression of the
recombinant protein was induced with 0.5 mM IPTG at 37degC for 3 h and harvest bacteria liquid.