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restriction endonuclease analysis

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restriction endonuclease analysis

[ri‚strik·shən ‚en·dō′nü·klē‚ās ə‚nal·ə·səs]
(cell and molecular biology)
A technique in which deoxyribonucleic acid (DNA) fragments obtained from digestion with restriction enzymes are compared to construct a restriction map showing the position of specific sites along a sequence of DNA.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive
Johnson et al., "Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein a gene sequence typing," Journal of Clinical Microbiology, vol.
Fidaxomicin versus vancomycin in the treatment of Clostridium difficile infection: Canadian outcomes
Small fragment restriction endonuclease analysis in epidemiological mapping of group A streptococci.
Genotyping of group a streptococcus by various molecular methods
Systematic relationships among waterfowl (Anatidae) inferred from restriction endonuclease analysis of mitochondrial DNA.
Evolutionary associations of brood parasitic finches (Vidua) and their host species: analyses of mitochondrial DNA restriction sites
In conventional restriction endonuclease analysis, chromosomal or plasmid DNA is extracted from microbial specimens and then digested with endonucleases into small fragments.
Molecular diagnostics of infectious diseases
By using restriction endonuclease analysis of the 65-kDa heat shock protein gene (1), we found that the isolate showed a pattern compatible with Mycobacterium simiae.
Iatrogenic Mycobacterium simiae skin infection in an immunocompetent patient
Restriction endonuclease analysis of DNA from two isolates of Chlamydia psittaci obtained from human abortions.
Chlamydophila abortus pelvic inflammatory disease
Development of a rapid and efficient restriction endonuclease analysis (REA) typing system for Clostridium difficile and correlation with other typing systems.
New Technology for Detecting Multidrug-Resistant Pathogens in the Clinical Microbiology Laboratory
The most widely used molecular typing methods include plasmid profiling, restriction endonuclease analysis of plasmid and genomic DNA, Southern hybridization analysis using specific DNA probes, and chromosomal DNA profiling using either pulsed-field gel electrophoresis (PFGE) or PCR-based methods (12,20).
Molecular Approaches to Diagnosing and Managing Infectious Diseases: Practicality and Costs
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