Prevalence of the most frequent high risk-HPV types by PapilloCheck[R] microarray and
restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP).
This restriction enzyme (Taq1) gave three different patterns with all isolated strains, isolated strains from pleural fluid and conjunctiva contained
restriction fragment pattern (rfp1) and isolated strains from nasopharynx contained rfp1 and rfp2 (Figure 2).
Tabaqchali, "Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and
restriction fragment length polymorphism analysis," Journal of Clinical Microbiology, vol.
In each case, DNA was double digested with the endonucleases and the resulting
restriction fragments were ligated with the double-stranded adapters.
Simulate the action of the enzyme by separating each DNA molecule into its resulting
restriction fragments (Figure 4).
From the
restriction fragments of the amplified ITS- rDNA region of the 51 isolates were obtained 1 to 3 restriction enzyme fragments, which two profiles for Acc I enzyme, 3 for Hpa II, 4 for Eco RI, HinfI, Rsa I, Alu I, Taq I and Hha I (Table 1 and 2).
No sequence-derived information is extracted from
restriction fragments, however, and no information is obtained on the order of fragment linkage.
Restriction fragments of PCR product of hsp65 and rpoB genes for reference strains of Mycobacterium and clinical samples
Restriction fragment of hsp65 gene
Restriction fragment sizes (bp) Reference strain/Clinical samples Hae III BstEII M.
A newer and rapidly developing way to characterize soil microbial communities is through the use of nucleic acid techniques such as terminal
restriction fragment length polymorphism (TRFLP) analysis.