However, if the number of herbicide tolerant plants was used as the base to calculate the transformation rate, the rate for
scarify treatment was 9.6% (number of Southern-positive seedlings, i.e., 3 per number of tolerant seedlings that were scarifying-treated, i.e., 31); and the rate for the puncture treatment was 15.5% (the number of Southern positive seedlings, i.e., seven per the number of tolerant seedlings that were puncturing-treated, i.e., 45).