Testicular cell number estimation: To estimate the total number of spermatogonia A (pale-staining nucleus with a fine "dusty" distribution of heterochromatin throughout the nucleus), B (with dense clumps of heterochromatin around the periphery of the nucleus), (24) Sertoli cells,
spermatocytes, round and long spermatids, and Leydig cells, a Nikon E200 light microscope (Tokyo, Japan) fitted with a 60x oil objective lens was applied at the final magnification of 1,640x.
The control negative group (200x; H&E) has normal cyto-architecture, reveal interstitial tissues (IT) and seminiferous tubules (ST) with sustentacular cells and the normal developing germ cells: spermatogonia (Sp),
spermatocytes I (SPI), spermatids (Spt), spermatozoa (Spz) with flagellum.
Score 5 denotes no spermatozoa or spermatids and many
spermatocytes; score 4 no spermatozoa or spermatids and few
spermatocytes.
Black perch < 90 mm SL did not have spermatozeugmata within the testicular tissues but all stages of
spermatocyte development were present (Fig.
In adult rats, Madhu, Sarkar, Biswas, Behera, and Patra (2011) observed reduction in seminiferous tubular areas, secondary
spermatocytes and nuclear diameter of Leydig and Sertoli cells after 45 days of exposure to 1 mg [kg.sup.-1] bw [day.sup.-1] of 3-MCPD.
5:
Spermatocyte abnormalities Primary rat
spermatocytes stained with an anti-SCP3 antibody were selected to quantify synaptonemal complex abnormalities by fluorescence photomicroscopy.
In a study, administration of 200 and 300 mg/kg to rats for 60 days showed a significant increase in the number of sperms, sertoli cells, and primary
spermatocyte compared to the control group.
Seminiferous tubules were labeled as type A, B, C according to the spermatogenetic activity variations between tubules; A: Tubule consisting of sertoli cells and spermatogonia, no
spermatocytes or spermatids; B: Tubule consisting of sertoli cells, spermatogonia and
spermatocytes, no spermatids; C: Tubule consisting of sertoli cells, spermatogonia,
spermatocytes and spermatids.
Radiation-induced chromosome aberrations in mouse
spermatocytes and oocytes.
Effects ofgamma rays on intact
spermatocyte cells, with complete cessation of the spermatogenesis process, was reported on peach fruit flies (Shehata et al.
After the meiotic division, a smaller secondary
spermatocyte is formed.