Control group Table 3: The number of different testis cell types (x[10.sup.6]) Groups
Spermatogonia A
Spermatogonia B Sertoli cell Control 0.83[+ or -]0.06 0.16[+ or -]0.07 2.7[+ or -]0.2 BD1/2 0.83[+ or -]0.05 0.13[+ or -]0.05 2.5[+ or -]0.1 BD1/4 0.84[+ or -]0.05 0.18[+ or -]0.05 2.7[+ or -]0.3 Groups Primary Round Long spermatocyte spermatid spermatid Control 12.1[+ or -]0.98 31.8[+ or -]2.49 30.9[+ or -]3.02 BD1/2 12.0[+ or -]0.80 31.0[+ or -]2.18 30.6[+ or -]2.66 BD1/4 12.4[+ or -]0.92 32.2[+ or -]2.20 32.1[+ or -]2.65 Groups Leydig cell Control 2.2[+ or -]0.02 BD1/2 2.5[+ or -]0.15 (**) BD1/4 2.1[+ or -]0.03 The values (n=5) are presented as mean[+ or -]SD.
(B) Group II seminiferous tubules of goat with loss of cellular cohesion of the germinal epithelium, cytoplasmic vacuolization of
spermatogonia and spermatocytes, sometimes with marginalized chromatin.
Op: primary oocyte, te: spermatic tissue, sp: spermatozoids, st: spermatocysts, sg:
spermatogonia.
Spermatogonia and spermatocytes were especially more positively stained.
Photomicrographs of the cross-sections of seminiferous tubules represent the stages of spermatogenesis of seminiferous epithelium:
spermatogonia type A (short arrows), primary spermatocytes at the pachytene stage (long arrows), spermatids at the zygotene stage (ellipse area) and spermatozoa (rectangular area).
In the testis, PLZF expression is restricted to As, Apr and Aal undifferentiated
spermatogonia, including SSCs (25).
Type A
spermatogonia at the basal lamina of seminiferous tubules, as well as primary spermatocytes in the leptotene and pachytene stages of prophase I, were also observed in the seminiferous epithelium.
Since rat spermatogenesis from
spermatogonia to mature sperm takes about 9 weeks, in our study, all rats were sacrificed under anaesthesia of 3% chloral hydrate (1 ml/kg) 9 weeks after the last GnRHant injection.
The small populations of SSCs are the ancestors of numerous differentiated and specialized cells including
spermatogonia, spermatocytes, spermatids, and mature sperms [2].
Spermatogonia are the only germ cell###0 (0%)###0 (0%)###0 (0%)###0 (0%)
Immature and resting phase (Figure 8a) were covered the numerous
spermatogonia and small seminiferous lobules was observed.
In a review, Parenti and Grier (2004) observed an evolutionary net on the teleosts testis morphology, through the organization of the seminiferous tubules and the distribution of
spermatogonia throughout the germinal epithelium.