Comparative evaluation of molecular assays for the identification of intestinal
spirochaetes from diseased pigs.
Treponema (43% vs 29%, belong to
Spirochaete), Ruminococcus (7% vs 5%, belong to Firmicutes), Roseburia (6% vs 5%, belong to Firmicutes), Pseudobutyrivibrio (6% vs 2%, belong to Firmicutes), and Anaeroplasma (3% vs 2%, belong to Tenericutes) were higher in Mongolian horses than in Thoroughbred horses.
Phylum % unclassified (derived from Bacteria) 14.48 Proteobacteria 12.91 Firmicutes 11.32 Actinobacteria 11.25 Bacteroidetes 10.16 Verrucomicrobia 8.20 Acidobacteria 5.73 Planctomycetes 4.34 Cyanobacteria 3.33 Aquificae 2.61 Nitrospirae 2.52
Spirochaetes 2.26 Thermotogae 2.10 Deinococcus-Thermus 2.04 Chlamydiae 1.82 Tenericutes 1.53 Dictyoglomi 1.26 Chloroflexi 0.73 Synergistetes 0.59 Thermodesulfobacteria 0.49 Chlorobi 0.33 Fibrobacteres 0.00 Gemmatimonadetes 0.00 Tabla 5: Abundancia relativa (%) de los principales filos identificados en muestras de suelos no contaminados del distrito Usquil, provincia Otuzco, departamento La Libertad.
More specific stainings were made in cases of suspected pathologies for which other histopathological stainings are indicated [24], such as periodic acid Shiff (PAS) staining and Grocott staining for fungi, Warthin-Starry (WS) for
spirochaetes and Ziehl-Neelsen (ZN) for mycobacteria.
The study of horses shows that their bacterial communities were dominated by Firmicutes (69.21% control group, 56.72% laminitis group) and Verrucomicrobia (18.13% control group, 27.63% laminitis group), followed by Bacteroidetes, Proteobacteria, and
Spirochaetes (Steelman et al., 2012).
Other dominant phyla identified in the gut of oysters, mussels, and clams include Tenericutes, Bacteroidetes, Actino-bacteria, Firmicutes, Chlamydiae, Fusobacteria,
Spirochaetes, Chloroflexi, Plantomycetes, and Verrucomicrobia (Fernandez-Piquer et al.
Based on this criterion, a total of twelve rare phyla were detected including Chloroflexi, Deferribacteres, Deinococcus-Thermus, Fusobacterium, Gemmatimonadetes, OP10, Planctomycetes, SR1,
Spirochaetes, TM7, Tenericutes, and Verrucomicrobia, which were detected in both stages of crop development.
Given the positive PCR assay result, a Warthin-Starry stain was requested on the histologically processed tissue biopsy and found to be positive for
spirochaetes (Fig.
The presence of
spirochaetes in human gastric mucosa.