Evaluation of the FASTPlaque TB assay for direct detection of Mycobacterium tuberculosis in
sputum specimens. Int J Tuberc Lung Dis 2002; 6: 635-640.
Four of these cases were unable to produce
sputum specimens (Table II).
The patient can be presumed to have NTM if a second
sputum specimen is smear-positive, NAA-negative, and has no inhibitors detected.
From July 1990 through August 1991, the inmate was transferred between three different state prisons, and
sputum specimens obtained during that time were predominately smear- and/or culture-positive.
In both instances, chest radiographs showed no new changes,
sputum specimens were negative for acid-fast bacilli (AFB) by microscopy and culture, and she was treated for presumptive Pneumocystis pneumonia and showed clinical improvement.
Sputum specimen from each participant was identified with a unique numbering system followed by A or B indicating soon after the patient wakes up and before any antiseptic mouth-wash use, respectively.
On HD 8 a
sputum specimen was sent for routine bacterial, fungal, and acid-fast cultures.
Direct smears were prepared from each
sputum specimen for microscopic investigation using AFB stain by the ZiehlNeelsen (ZN) technique (ST Reagensia Company, Jakarta, Indonesia) and interpreted according to standard guidelines.
Smear positive pulmonary tuberculosis defined as patient with at least two
sputum specimens positive for acid-fast bacilli (AFB) by microscopy, or radiographic abnormalities consistent with pulmonary tuberculosis with one
sputum specimen positive for AFB11.
However, the included criteria of
sputum specimen for microscopic examination were not described in this study.[sup][1]
The first aliquot of the
sputum specimen [2-3 mL in 14 mL BD Falcon [TM] centrifuge tube (Becton Dickinson, Franklin Lakes, NJ, USA)] was subjected to direct AFB smear examination and N-acetyl L cystiene (NALC)-sodium hydroxide (NaOH) decontamination method (final NaOH concentration 1%).
Sputum specimen volumes received from children for Xpert testing are shown in Table 1.