affinity chromatography

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affinity chromatography

[ə′fin·əd·ē ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.
References in periodicals archive ?
When used in mAb purification, this combination of attributes allows the hybrid purifier to provide superior product purity before the protein A affinity column, which in turn results in even greater purity downstream of the protein A operation, according to the company.
chartarum similarly revealed lysine-, cysteine-, and histidine-SG adducts after exhaustive digestion, affinity column enrichment, and MS analysis.
The proteins of interest are captured on each of the different resins, while the unbound plasma components flow through the column and form the feed to the next affinity column.
The serum IgG was purified by ammonium sulfate precipitation followed by protein A affinity column purification.
The affinity column was regenerated by washing with 5 mL of ultrapure water and 5 mL of 0.
americana that reacted to MAb38G6 that had previously been shown to be an allergen was prepared from the CR crude extract using MAb affinity column chromatography as previously described (Sookrung et al.
Kassel held the position of senior scientist at Glaxo Research Institute where he published one of the first papers coupling affinity column chromatography/MS for the identification of protein:ligand interactions.
The boronate affinity column that we used had a history of ~3500 injections.
The cytochrome P450 aromatase was purified from equine microsomes, after its separation from reductase, by successive chromatographic steps: concanavalin A-Sepharose 4B affinity column, diethyl amino ethyl-Sepharose CL-6B ion exchange, and hydroxyapatite-Sepharose 4B adsorption/partition columns (Moslemi et al.
The enzyme was eluted by 2 mol/L of NaCI/Tris buffer from the affinity column and then exhaustively dialyzed against TEAD buffer.
1c] whole blood application (Roche Diagnostics Corporation), Glyco-Tek affinity column method (Helena Laboratories), Glycosal [HbA.