agglutination reaction


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Agglutination reaction

A reaction in which suspended particles are aggregated or clumped. It occurs upon the admixture of another type of particle, a change in the composition of the suspending fluid, or the addition of a soluble agent that acts as a bridge between two or more particles. The reaction is a secondary one in that the process resulting in agglutination occurs after the primary antigen-antibody linkage has taken place.

The particles undergoing agglutination may be either unicellular or microscopic multicellular organisms (such as bacteria and parasites), individual cells of multicellular organisms (such as erythrocytes and lymphocytes), or artificial particles (such as beads of plastic, glass, or polysaccharide). The immunological specificity of agglutination depends upon the uniqueness of the reaction between a marker substance on one type of particle and a receptor on either another type of particle or a specific antibody in solution. The marker can be a usual biological component of the surface of the particle or blood group substance on red cells. It can be an enzymatically or a chemically modified chemical group on the surface of biological particles. It can also be an adsorbed or a chemically attached substance. The attachment can be to biological particles or artificial ones. The receptor can be a biological component of the particle, an attached antibody, or antibody in solution. A reverse reaction is one in which the antibody is attached to a particle and the addition of the antigen causes the mixture to clump. Inhibition of agglutination can also be used to test for antigens, especially of low molecular weight, in a manner similar to that for agglutination itself. See Antigen-antibody reaction, Immunoassay

agglutination reaction

[ə‚glüt·ən′ā·shən rē′ak·shən]
(immunology)
Clumping of a particulate suspension of antigen by a reagent, usually an antibody.
References in periodicals archive ?
The mixed agglutination reaction between latex particles and motile sperm proved the presence of sperm antibodies (IgG) on the sperm (12).
For the mixed agglutination reaction (MAR) test to be considered clinically significant, at least 50% of the motile spermatozoa need to be coated with antibodies.
Furthermore, all added erythrocytes has been diluted with PBS to all wells, shake it and micro plate hem agglutination reaction was observed at least after the 20 minute.
The agglutination reaction in a test tube was a pretty good reflection of what happens in life.
6) have used coplanar electric field to form chains of colloidal particles, thereby enhancing the rate of the latex agglutination reaction.
Methods for assessment of ASAB can be categorized into: 1) direct methods to detect the presence of ASAB on sperm (sperm mixed agglutination reaction [sMAR], immunobead test [IBT]) or; 2) agglutination tests using donor sperm (gelatin agglutination test, tray agglutination test [TAT], radiolabeled agglutinin assay).
When they are bound by antigen that may be present in the specimen, an agglutination reaction occurs that is visible with the naked eye.
The absorbance produced by the latex agglutination reaction increased with decreasing pH of reagent 1, but fibrinogen in plasma precipitated at pH values below 6.
2 List of Figures Figure 1: Colorectal Cancer Staging 24 Figure 2: Trends in Colorectal Cancer Incidence, 2010-2019 44 Figure 3: Example of a Guaiac Fecal Occult Blood Card: Immunostics Hema-Screen 48 Figure 4: Fecal Occult Blood Test Lateral Flow Device Architecture 52 Figure 5: Biohit Colonview (example of immuno-FOB test) 53 Figure 6: Beckman Coulter Hemoccult-ICT (example of immuno-FOB test) 54 Figure 7: Veda Labs Easy Reader Immuno-FOB Test LFD Reader 60 Figure 8: OC-Hemodia Agglutination FOB Test (manually performed) 65 Figure 9: OC-Sensor/Diana FOB Test Sample Cartridge 66 Figure 10: OC-Sensor/Diana FOB Test Instrument 67 Figure 11: Fujirebio HemSp/MagStream HT Magnetic Agglutination Reaction 68
Although the main purpose here is to describe assay features and analytical performance, we also describe results of work done to shed light on the mechanism of the agglutination reaction used.
These include tray slide sperm agglutination, mixed agglutination reaction, sperm immobilization, sperm cytotoxicity, ELISA, indirect immunofluorescence, radiolabeled antiglobulin, immunobead binding, and flow cytometric sperm antibody.