agglutination reaction

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Agglutination reaction

A reaction in which suspended particles are aggregated or clumped. It occurs upon the admixture of another type of particle, a change in the composition of the suspending fluid, or the addition of a soluble agent that acts as a bridge between two or more particles. The reaction is a secondary one in that the process resulting in agglutination occurs after the primary antigen-antibody linkage has taken place.

The particles undergoing agglutination may be either unicellular or microscopic multicellular organisms (such as bacteria and parasites), individual cells of multicellular organisms (such as erythrocytes and lymphocytes), or artificial particles (such as beads of plastic, glass, or polysaccharide). The immunological specificity of agglutination depends upon the uniqueness of the reaction between a marker substance on one type of particle and a receptor on either another type of particle or a specific antibody in solution. The marker can be a usual biological component of the surface of the particle or blood group substance on red cells. It can be an enzymatically or a chemically modified chemical group on the surface of biological particles. It can also be an adsorbed or a chemically attached substance. The attachment can be to biological particles or artificial ones. The receptor can be a biological component of the particle, an attached antibody, or antibody in solution. A reverse reaction is one in which the antibody is attached to a particle and the addition of the antigen causes the mixture to clump. Inhibition of agglutination can also be used to test for antigens, especially of low molecular weight, in a manner similar to that for agglutination itself. See Antigen-antibody reaction, Immunoassay

agglutination reaction

[ə‚glüt·ən′ā·shən rē′ak·shən]
Clumping of a particulate suspension of antigen by a reagent, usually an antibody.
References in periodicals archive ?
27) Cross-linking (ie, when 2 immunoglobulins are bound across 2 different cells) is the necessary endpoint for the agglutination reaction, but flow cytometry only requires the RBC membrane to be coated with immunoglobulins.
2] Table 2: Results of oxidants in the agglutination reaction.
Regarding the 10% prevalence of ASA (IgG) in infertile men who have no other problems, sperm mixed agglutination reaction (MAR) should be considered as a routine test in the semen analysis of patients with varicocele (23).
The concentration of HA with a dilution 1/64 showed that the agglutination reaction between the protein immunogenic VNN 45 kDa and erythrocytes of Humpback grouper showed levels of sensitivity to concentrations are 0,015625.
The patient had positive Wright serum agglutination reaction at titer of 1/320.
When they are bound by antigen that may be present in the specimen, an agglutination reaction occurs that is visible with the naked eye.
6) have used coplanar electric field to form chains of colloidal particles, thereby enhancing the rate of the latex agglutination reaction.
The absorbance produced by the latex agglutination reaction increased with decreasing pH of reagent 1, but fibrinogen in plasma precipitated at pH values below 6.
Although the main purpose here is to describe assay features and analytical performance, we also describe results of work done to shed light on the mechanism of the agglutination reaction used.