The results of measurements in the baseline aliquots
and in those containing increasing levels of cell-free hemoglobin were evaluated by Student's paired T test and direct comparison with the desirable specifications for total error, as recommended by Ricos et al (11).
At three separate institutions, an hCG-negative plasma sample with undetectable hCG on the Unicel DxI was identified, transferred to an aliquot
tube, spiked with a leukocyte-enriched buffy coat (125 [micro]L added to 700 [micro]L plasma), and returned to the original PST.
Each paired plasma sample was removed from the cells within 3 minutes of each other and given a unique identification designation for each separate aliquot
Castlestone said the weightings were selected by the investment manager drawing on experience gained from managing the Aliquot
Gold Bullion Fund (BVI) since 2003.
Dissolve trypsin at a 1mg/ml concentration in double distilled water and freeze small aliquots
The amount transfused with each aliquot
ranged from 5.
The DNA-containing pellet was hydrolyzed in 5% trichloroacetic acid for 15 min at 90[degrees]C and resedimented, and an aliquot
of the supernatant solution was counted for radiolabel.
A fifth aliquot
with no humectant nor added water served as the control.
of transformer oil from the drum of the Exxon Univolt 60 transformer oil was examined for traces of PCBs as would be evident by capillary gas chromatography with electron capture detection (GC-ECD).
Feedings consisted of a per capita 25 mg aliquot
of a finely ground 1:1 mixture of rabbit chow and fish food flakes, i.
Detection of SARS-CoV RNA by RT-PCR confirmed by a second PCR assay, by using a second aliquot
of the specimen and a different set of PCR primers
of the supernatant was removed and evaporated to dryness under a stream of nitrogen (Meyer N-evap); the residue was redissolved in 12 ml methanol:water (80:20 v/v); and ce ntrifuged as above.