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All other canine and equine viruses used in the hemagglutination inhibition (HI) assay were grown in 10-day old embryonated chicken eggs from which allantoic fluid was collected after 72 h and stored at -80[degrees]C.
Briefly, virus-infected allantoic fluid was diluted (1:1,000) into continuously stirred, chlorinated, chlorine demand-free phosphate buffer (0.
One group was inoculated with normal allantoic fluid as a negative control.
Infective allantoic fluid was diluted 1:10 in sterile phosphate-buffered saline (PBS), and 0.
RNA extraction and cDNA synthesis: Viral RNA was extracted from 250 [micro]L of all the clinical specimens and from 140 [micro]L of the virus-containing allantoic fluid (reference strains) using commercially available QIAamp Viral RNA Mini Kit (Qiagen GmbH, Hilden, Germany) as per manufacturer's instructions.
Allantoic fluid (AF) from two eggs selected randomly was harvested at an hourly interval and stored at -70[degrees]C.
Recent Highlights * Sales of Synagis(R) increased 12 percent to $472 million * FluMist supplements approved by FDA to reduce normal allantoic fluid (NAF) and eliminate FreezeBox storage requirements * Ex-U.
Allantoic fluid from the injected eggs agglutinated 0.
3 showed embryonic mortality before 36 h in all inoculated eggs (4/4) and HA activity was detected (2 (8) and 2 (11)) in the allantoic fluid.