(LC-MS grade) was purchased from Sigma-Aldrich Chemie GmbH.
Denoxium is a solution of ammonium formate
and urea and it has a freezing point of -30oC.
Kemira has already demonstrated its ammonium formate
-based DENOXIUM(TM) concept to all the leading vehicle manufacturers in Europe and the reception has been enthusiastic.
Kemira has developed Denoxium, a mixture of urea, ammonium formate
and water--as a fully compatible and interchangeable replacement for urea in such systems.
The enzyme activities for GAA, GLA, and IDUA were compared in sodium formate and ammonium formate
buffers at pH 4.
We obtained acetonitrile and methanol from Carlo Erba, dichloromethane and isopropanol from Prolabo, and formic acid and ammonium formate
To each well we added 70 /,L of 5 mmol/L ammonium formate
in methanol-chloroform (3:1 by volume), and the sample was infused into the mass spectrometer.
Cortisol and ammonium formate
were purchased from Sigma, and [d.
1 cm) packed with 5-[micro]m diameter particles of Hypersil silica (Agilent Technologies) and equilibrated with 25% solution A (15 mmol/L ammonium formate
, pH 3.
4]Cl, disodium EDTA, formic acid, sodium metabisulfite, ammonium hydroxide, ammonium formate
, 1-octanol, and glacial acetic acid were purchased from Sigma.
The mobile phase consisted of 850 mL/L methanol and 150 mL/L ammonium formate
The mobile phase consisted of 50 mmol/L ammonium formate
buffer (pH 3):methanol (73: 27, by volume).