ampholyte


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ampholyte

[′am·fə‚līt]
(chemistry)
An amphoteric electrolyte.
References in periodicals archive ?
5M Urea, 4% NP-40, 5% (v/v) 2-mercaptoethanol, 2% v/v ampholytes of pH 5-8 and pH 3-10.
5% bio-lyte ampholyte (3-10, Bio-Rad), bromophenol blue (a few grains).
Isoelectric focusing of the hemolysate was performed on a polyacrylamide gel plate containing carrier ampholyte (pH range 6-8 and 3.
In this study, three sheets of cellulose acetate membranes that had absorbed 100 g/L ampholyte Sepaline (pH 3.
The ampholyte solution consisted of a mixture of Pharmalyte pH 6-8 and Pharmalyte pH 7-9 (3:1, by vol), at a final concentration of 20 mL/L in a 4 g/L methylcellulose solution.
The isoelectric focusing (IEF) was performed without ampholytes and glycerol according to the manufacturer's instructions for peptide focusing.
Although gel-based techniques for protein separation are tremendously powerful, the observation of protein bands reflects a complex set of interactions between protein, ampholytes, buffer composition, gel composition, and pore size.
The isoelectric focusing of proteins can be determined using immobilized pH gradients or ampholytes.
Proteins are ampholytes, exhibiting amphoteric behavior owing to the presence of both positively- and negatively-charged side chain groups distributed along the length of the molecule and at its ends.
0 ml ampholytes (pH gradient 4-5; Crescent Chemical, Hauppa, NY), 4.
DNA) and low-molecular-weight ampholytes and strong protein associations.