With respect to the results listed in Table 2, the orange and grape juices of the five food companies, at both exposure times considered, induced significant formation of mitotic spindle changes, represented in this study by colchicine metaphase and anaphase
and telophase bridges, proving to be genotoxic, and chromosome breaks, characterized by the formation of micronuclei.
14) Vukovic and Sofradzija (1989) examining the genotoxic action of Lorsilan, Librium, a Mepbromat and Apaurin came to the conclusion that the first three have similar effects, and is manifested by lowering the mitotic index, the advent of C-mitosis, abnormal metaphases and anaphases
The chromosomes in precocious migration in metaphases I may have originated from univalents in diakinesis or have been a result of precocious terminalization of chiasma and the late terminalization of these chiasmas makes the laggard chromosomes in anaphase
I (SOUZA-KANESHIMA et al.
Normal Multipolar fragments Anaphase Anaphase
1 + - - 2 + - - 3 + - - 4 + - - 5 + - - 6 + - - 7 + - - Sample No.
The cells were recorded as normal or aberrant in the different stages of the cell cycle namely: interphase, prophase, metaphase, anaphase
were typical, well organized and others were amply disordered, with chromosome bridges (Figure 5a, b).
lyoni, with n = 11 or n = 12 = 11 + X, confirmed the regular reductional segregation of all the chromosomes in the preceding anaphase
I and the meta- and submetacentric morphology of the chromosomes (Figs.
As the genetic material move to the polar region of the cell, at anaphase
, these centromeres are pulled to opposite directions, creating the chromosome bridge.
No difference in frequencies of abnormal metaphases and anaphases
was found when compared to C5-A6-T6 and C5-A8-T8 cells (Table 2).
Cell changes observed when combining the flavorings were micronuclei colchicine metaphase, anaphase
and telophase bridges.
The aberrations observed were micronuclei, gene amplifications, anaphase
bridges and telophase bridges.