annealing temperature


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annealing temperature

[ə′nēl·iŋ ‚tem·prə·chər]
(thermodynamics)
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CR], goes up moderately upon the increase of the annealing temperature, as shown in Fig.
However, amplification of target decreased at 57[degrees]C and there was no amplification at the annealing temperature of 60[degrees]C at which no difference was detected in the samples from China (Figure 2).
Twenty-four loci were amplified consistently across the tested specimens and were all labeled with fluorescent dyes for further evaluation and optimization of annealing temperature.
In other words, increase in the annealing temperature leads to more random arrangements of edges within the distance r in the sense of their disorientation angles.
In this laboratory exercise, students use two sets of primers to determine optimal annealing temperature on PCR product formation to optimize for efficiency of amplification.
25) Films with good crystallinity were obtained at an annealing temperature of 650[degree]C.
It has been shown to reduce annealing cycle time by more than 25% and annealing temperature by more than 25[degrees] F.
In many cases, the control soak period is often built into a heat treatment cycle as a safety buffer to ensure that the entire load is uniformly heated to its required annealing temperature.
If the annealing temperature is too high, the primers will not anneal efficiently, resulting in no amplification or poor yield; if it is too low, primer mismatches and non-specific amplification may occur, and yield may be diminished.
It can be seen that when the annealing temperature of the MLC is increased ( = 50%), the [H.