antisense

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antisense,

DNA or RNA manipulated in a laboratory so that its components (nucleotides) form a complementary copy of normal, or "sense," messenger RNA (mRNA; see nucleic acidnucleic acid,
any of a group of organic substances found in the chromosomes of living cells and viruses that play a central role in the storage and replication of hereditary information and in the expression of this information through protein synthesis.
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). Antisense techniques are used to deactivate disease-causing or undesirable genes so that they cannot produce harmful or unwanted proteins. (Conventional drugs bind directly with disease-causing protein molecules, but their imperfect specificity may lead them to bind with other protein molecules, resulting in unwanted side effects. Antisense molecules are extremely specific.) In some applications of this technique, the antisense nucleic acid segment is inserted into an inactivated or nonvirulent virus, then introduced into the cell. The antisense segment pairs with the mRNA, preventing the synthesis of protein by the mRNA. Antisense has applications in agricultural biotechnology, where it has been used to deactivate the gene that causes softening in tomatoes, and in medicine, especially in cancer and antiviral therapy. See also gene therapygene therapy,
the use of genes and the techniques of genetic engineering in the treatment of a genetic disorder or chronic disease. There are many techniques of gene therapy, all of them still in experimental stages.
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antisense

[¦an·tē′sens]
(genetics)
A strand of deoxyribonucleic acid having a sequence identical to messenger ribonucleic acid.
References in periodicals archive ?
Rational design of point mutation-selective antisense DNA targeted to codon 12 of Ha-ras mRNA in human cells.
Three known RNA polymerases exist in eukaryotic cells, and all RNA polymerases require the presence of antisense DNA as a template.
Working with S2 and S3 alleles in petunias, Kao first triggered a loss of self-recognition by using bacteria to insert antisense DNA, which mirrors normal DNA, into the flower's genome.
These studies showed, for the first time, that an antisense DNA analog can inhibit bacterial growth in animal infections.
In addition, antisense technology is being developed by pharmaceutical companies, which use antisense DNA as a therapeutic means to inhibit the activity of genes that cause certain diseases.
In contrast, antisense DNA sequences are orders of magnitude more precise in their targeting ability.
A study using a modified form of the herpes simplex virus to introduce an antisense gene into sensory neurons in the hindpaws of mice demonstrated that the antisense DNA blocked the normal response to an inflammatory stimulus and prevented the development of hyperalgesia, or excessive sensitivity and pain.