Because the latter antiserum
tolerates the loss of only a few amino acids from the N-terminal end before losing immunoreactivity, it is likely that these components have lost 1 or 2 amino acids from the N-terminal end.
Antigen is loaded in trough to get dispersed in the gel by electro diffusion process and in prepared wells antiserum
of IgG and total serum is added for diffusion which shows a difference in the zone of precipitation.
Four hundred [micro]l incubation buffer were added to a tubes for non specific binding (NSB), and 300 [micro]l were added to the other tubes with 100 [micro]l diluted antiserum
Venom and antiserum
were mixed and incubated at 37 [degrees]C x 1 h, centrifuged at 2000 rpm x 10 min and supernatant was injected (i.
5% nonfat dry milk) were added to the wells of antigen-coated microtiter plates before addition of the anti-HDI-KLH antiserum
It is unknown if this pattern was due to differences in the antiserum
specificity of manufacturers or to values based on the detection of C3 versus C3c.
As the elk antiserum
used in this analysis was known to elicit a positive reaction to bison, the weak positive reactions to this antiserum
were not unexpected.
will bind to the protein molecule against which it was raised, forming an extended lattice (Benjamin et al.
Because closely related species have similar blood proteins, the antiserum
for, say, elk can react with blood from a deer or cow.
We are delighted to have the opportunity to have our BSE antiserum
and test protocol evaluated in close cooperation with CVL," said Jurek S.
In the serum samples, however, antiserum
against the epitope NT-[proBNP.
Finally, in vitro and in vivo cytotoxicity of the polyclonal antiserum
was determined using primarily cultured pig adipocytes and piglets, respectively.