MPCM and SBFSEM phenotyping of rice mutants compromised in arbuscule
development will provide a novel insight into the complex molecular mechanisms that underpin cellular morphogenesis during plant endosymbiosis.
formation on the intraradical hyphae or hyphal coils varied from very limited (e.
represents a dead end in the growth of AM fungi (Bonfante 1995) as they senesce and collapse after 4-10 days of symbiosis (Sanders 1977) and thus the plant cell recovers its original morphology (Jacquelinet-Jeanmougin 1987).
The microscope was focused through the entire root segment and if an arbuscule
or vesicle was detected, the root segment at the intersection was scored as infected.
2010) recorded highest AM colonization (M = 78%) and arbuscule
richness (A = 76%) in Thelypteris patens (Sw.
If an arbuscule
or vesicle was found at an intersect, that intersect was considered infected.
Family Species Mycorrhizal structures Arbuscule
Asteraceae Ageratum conyzoides (L).
abundance (A%) is an estimate of arbuscule
richness in the whole root system (Trouvelot et al.
Only one arbuscule
within each cell was observed (Fig.
Moreover, it should also be noted that the number of arbuscules
present in the arid climate is sometimes more important than in sub-humid climate, this demonstrates the importance of endomycorrhizae role, especially arbuscules
that represent sites of functional exchanges between the endomycorrhizal fungus and roots of the host plant .
Roots were observed under a dissecting microscope and scored for presence or absence of hyphae, vesicles, or arbuscules
along the root--grid-line intersects.
Root colonisation by AM fungi was defined by the presence of arbuscules
and hyphae within roots and expressed as percentage of root length; 6 repeated measurements were taken on each sample, redispersing the sample between each measurement.