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Kramer and his collaborators first evaluated avidin as a biopesticide in the early 1990s.
They plan eventually to incorporate the avidin or streptavidin gene into plants and test to see whether the plants resist the insects.
Binding was detected by using monoclonal antibody 2G3 against RHDV, biotinylated anti-mouse IgG, horseradish peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole substrate with hemalum counterstaining, as described (2).
ITC] being the cardiac troponin complex containing all 3 subunits of I, T, and C) assays, bt-8I-7 mAb was immobilized on an avidin sensor as the capture antibody.
We purchased antimurine IL-4 monoclonal antibody (mAb) clone 11B11 from Hazleton (Hazleton, PA, USA); biotin conjugated anti-IL-4 mAb clone BVD6-24G2 from Pharmingen (San Diego, CA, USA); horseradish peroxidase (HRP)-conjugated avidin from ICN Pharmaceuticals Inc.
Now researchers in the basic and clinical sciences and protein production have additional tools for immunoassay design and interaction experiments to complement the original dotLab([R]) Avidin Sensors.
More recently, we developed several types of biotargeting trifunctional nanospheres by coembedding QDs and nano-y-Fe,O, particles into poly(styrene/acrylamide) copolymer nanospheres and then covalently coupling different biological reagents, such as folic acid, biotin, avidin, and antibody, onto the surfaces of the nanospheres (24,29-31).
The select targeting is accomplished with a "pre-targeting approach" involving a first step of localizing a biotin or avidin molecule to the diseased area, followed by the enhanced binding of the matched avidin or biotin, respectively, which bears the diagnostic or therapeutic agent.
Binding of the aptamers to the target protein forms a sandwich complex, which is immobilized onto a microplate via avidin.
To complement its new instrument, Quadraspec has launched the Assay Development Kit (ADK[TM]), 96 well discs with "generic" capture agents such as Avidin, Protein A/G and Anit-mouse Fc antibodies.
Methods for immobilizing proteins on gold film, which have been investigated for years, include the SPA method (15, 23), the avidin and biotin method (24, 25), and the self-assembled monolayers method (26, 27).
announced today publication of two studies regarding the potential use of its Single Amino Acid Chelate (SAAC(TM)) technology for linking biotin and avidin to two common nuclear imaging and radiotherapeutic isotopes, technetium and rhenium.
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