base stacking


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base stacking

[′bās ‚stak·iŋ]
(cell and molecular biology)
The orientation of adjacent base pairs such that their planes are parallel and their surfaces almost touch, as occurs in double-stranded deoxyribonucleic acid molecules.
References in periodicals archive ?
The patent covers the application of base stacking technologies that use specific types of oligonucleotides, both capture and probe oligonucleotides, to bind and discriminate nucleic acid sequences of differing lengths.
When the terminal nucleotides are adjacent, the internal energy of base stacking increases, thereby increasing the stability of the complex as compared to complexes formed with lower stability, either because the sequences are mismatched or the terminal nucleotides of the capture and probe oligonucleotides are not adjacent.
This method takes advantage of the premise that base stacking stabilizes DNA hybridization such that matched reporter binding is preferentially more stable than mismatched reporter binding (4).